Civil management is an important aspect in the success of organization Essay

Civil management is an important aspect in the success of organization - Essay Example It is in this regard that this paper is written. Management of organization is becoming an increasingly daunting task in the contemporary times, this is due to the ever advancing business world and business technology leading to ambiguity, uncertainty and complexities of organization. Effective internal management of an organization demands that people and facilities in the organization are managed well. This introduces the concept of organizational behavior which involves how people within the organization react to changes, decisions and practices of management by managers. It is also important to have human skills in order to incorporate the views of other people and manage them well; this will also ensure that the people work towards a common goal within the organization (Pitsis, Clegg & Kornberger, 2011, p 29). Therefore, critical management is determined to harmonize the workplace, through ensuring that the employees and facilities are managed well and working conditions improve d. In order to ascertain this, managers need to rely on management theory and practice in order to deal with the uncertainties of organizations. These management theories and practices deal with construction of models based on observations, to try and manage business activities (Carr, 2000, pp. 210). In addition, the practices of management also deals real life experiences of the manager within an organization. This paper therefore seeks to critically evaluate the impact of uncertainty on how the organization is managed through culture leadership, structure, control, and empowerment. Additionally, the paper will demonstrate a critical understanding of management practice and theory, ability to identify and evaluate vital assumptions in the ideology of management, and analyze and evaluate organization and management theory and appraise the application of management theory in organizational contemporary contexts. Uncertainties of organization Organizations are faced majorly by uncerta inty challenges; this impact on their production and performance levels. Uncertainty as an element in managing organizations involves risks and unpredictability of the organization’s future events. This may include surprises in employee performance which could either lead to losses or profits within the organization (Renn, Klinke & Asselt, 2011, pp. 3). As a newly recruited manager assigned the duty of dealing with uncertainty, ambiguity, and complexity situations, one is expected to identify and evaluate the key assumptions of management ideology and apply the management and organization theory in handling the concerns in the contemporary context. A junior manager is expected to use the â€Å"box paradox†, in order to come up with new management strategies that will ensure organizational growth and retain the identity and founding values of the organization (Clampitt & Williams 1999, p 6). The junior manager will have to â€Å"think outside the box† and acknowl edge the constraints of the â€Å"organizational box† in order to achieve growth and development of the organization and handle the complexity, ambiguity and uncertainty prevailing in the organizatio

Honor, Virtue, and the Paradox of the Old South (Antebellum period and Essay

Honor, Virtue, and the Paradox of the Old South (Antebellum period and most specifically 1800 to 1864) - Essay Example re expected to wear beautiful gowns rich in cloth and engage in gentile activities that didn’t stress them too much or place them in the harsh sun for excessive periods of time. Black people were barely considered at all and were, at best, seen in the background the scenes in one’s head, with a serving tray or assisting a woman with her dressing rituals. Even when daily life did not match up with these ideals, the belief was that they were working toward them and that the Southerner was the final champion of the highest ethical standards known to man. However, very few of these ideals were actually true. While there were numerous planters that may have fallen within these parameters, they were only able to do so by exploiting the people around them. The South survived on the backs of its women and slaves in a way that it rarely acknowledged openly, introducing a tremendous paradox between how they saw themselves as compared to how they really lived. This is most easily understood in the stories of the ‘servant’ members of this society, the women and slaves/former slaves. Following the end of the Civil War, the United States went into a period of rebuilding and redefinition in many respects. One of the ideas that developed during this period among the middle class of the country was the idea of women as the center of the home. Scholarship on this issue brings into focus some of the issues of class and reproduction women faced during the antebellum period. Through the virtues of piety, purity and submissiveness, the woman was defined first as a pious and pure daughter and sister and then as a submissive wife within the confines of the male protector, making her suitable only for a domestic role. Her prime motive following marriage was to provide for the hearth, meaning food, clothing, children and all that was necessary for the continuance of the family line. When it came to marriage and having children, it was said â€Å"Let no caprice or

Separation of Histone Protein

Separation of Histone Protein For estimating protein mixture qualitatively most widely used method is SDS-Polyacrylamide gel electrophoresis (SDS-PAGE). According to size of the protein, this SDS-PAGE is separate the protein and purification of protein is to be monitored by this method, and relative molecular mass of protein can also be determined. In this SDS-PAGE anionic detergent is SDS. Before loading the sample, the samples are boiled for 5minutes, that contain s SDS and à ¯Ã‚ Ã‚ ¢Ãƒ ¯Ã¢â€š ¬Ã‚ ­mercaptoethanol in the buffer. While boiling the sample the SDS act to denature the protein and where à ¯Ã‚ Ã‚ ¢Ãƒ ¯Ã¢â€š ¬Ã‚ ­mercaptoethanol decrease the disulphide bridges of the protein that are holding tertiary structure of protein .by this denature process the protein get fully denatured and form a rod shape structure with negatively charged molecules of SDS throughout polypeptide chain. Every couple of amino acids binds with one SDS molecule on average. Due to the negatively charge SDS the structure remain s as rod like. So repulsion take place between the negatively charge on proteins and no folding occurs and remains rod shape. In the sample loading buffer, contains bromophenol blue and sucrose or glycerol. The bromophenol blue is helpful in monitoring the sample, when electrophoresis running and glycerol give density to the sample that can settle at the bottom of the well on stacking gel. The samples are loaded on the electrophoresis gel, which is made up of two gels .the lower gel is main separating gel and upper gel is stacking gel. This stacking gel helps in loading the sample into wells and had large pore size. Where protein sample moves freely and makes the protein sample concentrate and forms sharp band and enters into main separating gel with effect of electric field. Here isotachophoresis take place. The glycinate ion which is negatively charge has lower mobility than SDS-proteins molecule in running buffer than cl- ion in stacking and loading buffer. At the higher field strength both cl- and glycinate travel at same speed. So these ions and protein adjust those concentrations. The separating gel has higher PH environment, once glycine receives it become highly ionised state and mobility increases. By this the cl- and glycinate leaves the SDS-protein molecule. Now the SDS-Protein molecule moves towards the anode in separating gel by the effect of electric field. Here the protein having smaller size moves faster and reaches to the bottom of the gel than protein having larger size, with the help of bromophenol blue dye we can indicate the electrophoresis front because smaller particle unretarded the dye colour. When dye comes bottom of the gel then turned off the current, remove the gel from the sandwich properly and stained with coomassie brilliant blue and then by using destainin g solution, gel is washed. Depending on the protein size the preparation of polyacrylamide gel is used like 15%, 10% and 7.5%. By the help of the standard protein the mobility of unknown can be calculated by using calibration curve. In SDS-PAGE the protein should give single band, then that protein is said to be pure. So for purification protein process SDS -PAGE is most widely used. To the cluster of eight histone protein (H1-H8) DNA is wounded around. By the help of histone and DNA chromatin is made. The regulation of expression of genes and organisation of DNA is done by the help of histone proteins. Due to histone protein modification we can keep the genes active or silent and modifications are like methylation and acetylation. The transcription factors take place by the modulate accessibility of DNA by histone modification. DNA access might blocked by histone methylation to transcription factors. Electrostatic interaction might change due to histone acetylation in chromatin and allows transcription after opening up DNA. In blood cells development in chicken the principle of the histone modification is clearly demonstrated. In the transition the structural and functional role is played by histone protein between the states of active and inactive chromatin.high degree of conservation consists in histone . This is due to structural maintained constraining the e ntire nucleosomal octameric core. In the gene regulation and epigenetic silencing the diverse role play by a histone proteins.DNA replication, repair, transcription and recombination are influenced by the post translational modification, interactions with chromatin remodelling complexes and histone variants. DNA is packed in the nucleus and forms a complex called chromatin. The first level of chromatin organization is represented by the nucleosome core particles. The octameric core is composed of 146-147 bp of DNA that are tightly wrapped around two copies of histone H2A, H 2B,H3 and H4. Nucleosome cores are associated with linker histone H1 and separated by variable length of linker DNA. Core histone internucleosomal interactions are mediates by composing packed nucleosome arrays to start helical model. Due to the presence of histone fold domain the core histone are characterised and variable lengths of N-TerminaL tails are extensive subjects for post translational modifications. T he epigenome are the component of post translational modifications hence that includes protein connected to its gene and changes in DNA occur. For regulation of gene expression the epigenetic mmodifications are act as switches. DNA and histones are its chemical modifications .which does not disturb the sequence changes to DNA. The organisms reveal a variety of striking similarities despite histone tail and core variation due to characterization of structural nucleosome core particles. Using structural information they reanalysed histone fold domain variably sequence in a novel fashion. The variable pair of histone protein are H2A and H 2B and the conserved one are H4 and H3. In eukaryotes histone proteins are associated with DNA and are positively charged, this is due to presence of positively charged amino acids like lysine and arginine . H 1, H2A, H 2B histone are rich in lysine and H3 , H4 are rich in arginine. Each nucleosome consists of 8 histone proteins. Around one nucleosome to another nucleosome 200bp is present in DNA. In a circle of 1 nucleosome 146 bp are present. Where 54 BP are present in connection link of DNA between 1 nucleosome to another nucleosome. In nucleosome H1 histone is absent.here linker DNA connects two nucleosomes and H1 protein present in linker DNA. H1 protein takes an active role in formation of eukaryotes and heterochromatin. Genetic and epigenetic changes both involved in breast carcinogenesis and it is a multi step process. Epigenetic is a change that observed in gene expression in both reversible and heritable by the gene sequence without alteration. In cancer that influence the two major epigenetic changes are DNA methylation and histone modification interactions is well orchestrated. Malignant and premalignant breast neoplasm is methylated by involvement of several genes in metastasis, proliferation and antiapoptosis. In breast cancer treatment with other systemic therapies, histone deacetylase inhibitors become synergistically an important class of drugs. Potentially reversible processes are epigenetic changes and for finding novel therapies and refined diagnostic of breast cancer many efforts has been done for understanding the mechanism. MATERIALS AND METHOD: 30% W/V Acryl amide /Bis acrylamide Tris Hcl 3.0M, PH = 8.8 (lower gel) Tris Hcl 0.5M, PH =6.8 (upper gel) Bio-rad mini protean tank TEMED Ammonium persulphate (APS 25%W/V) Running buffer Bromophenol blue Sample buffer Coomassie blue stain Human recombinant proteins H4,H3.3, H2B, H2A EXPERIMENTAL PROCEDURE: SDS -PAGE GEL PREPARATION: PREPARATION OF GEL CASSETTE SANDWICH: The casting frame is taken and place on the flat surface. Select the glass plates to make a sandwich and place the short plate on the spacer plate and fix the casting frame to make sandwich. Fix the casting frame to the stand and the sandwich glass plates on the gray rubber gasket. Then checked the sandwich plates with distilled water to ensure any leakage occur. Prepare the resolving gel into a beaker without adding TEMED and APS. Add TEMED and APS into the prepared resolving gel and mix the solution homogenously and immediately pour the mixed solution into the sandwich plates, more than half of the glass plates. Allow the resolving gel for 35-40 minutes to get gel polymerised. Wash the resolving gel with distilled water and discard the water from sandwich, dry the inner surface by using filter paper. Prepare the stacking gel into another beaker without adding the TEMED and APS. Added TEMED and APS and mix equally and pour it on the top of the resolving gel and gently place the comb on the top of the stacking gel. Then leave the stacking gel overnight for its polymerization. RESOLVING GEL AND STACKING GEL PREPARATION: Resolving gel: acrylamide/bis-acrylamide 10.0ml,3.0M Tris /Hcl (PH=8.8) 3.75ml,dH20 15.8,10% SDS 0.3ml,TEMED 0.015, Ammonium Per sulphate 0.15. Stacking Gel: Acrylamide/bis acrylamide 2.5ml,0.5M Tris /Hcl (PH 6.8) 5.0ml,dH20 12.26ml,10% SDS 0.2ml,TEMED 0.015ml,Ammonium persulphate 0.04ml. Separation of H2A/H2B/H3.3/H4 Human Recombinant Protein using 1D SDS-PAGE Gel . After overnight polymerisation taken out the comb carefully and well are washed with running buffer. Remove the gel sandwich from the casting stand and allow to place them in the electrophoresis tank placing short plate facing inwards. Fill the gel electrophoresis tank with running buffer up to halfway between inner chamber i.e. 125ml and in the mini tank add 200ml of running buffer. SAMPLE PREPARATION AND LOADING: Taken the sample of histone protein of à ¯Ã¢â€š ¬Ã‚ ±Ãƒ ¯Ã¢â€š ¬Ã‚  Ãƒ ¯Ã‚ Ã‚ ­l and added into the sample buffer of 20à ¯Ã‚ Ã‚ ­l eppendorf tube. The protein samples are labelled to each tube. The histone protein samples are heated to 100o c for 2 minutes in hot block and at room temperature allow cooling down. Now samples of histone proteins are allowed to load into the well of 20à ¯Ã¢â€š ¬Ã‚  Ãƒ ¯Ã‚ Ã‚ ­l of each sample with the help of loading gel tips and while loading, load the sample carefully and slowly without air bubbles and allow the sample to settle down at the bottom of the wells. Taken molecular marker of 2à ¯Ã¢â€š ¬Ã‚  Ãƒ ¯Ã‚ Ã‚ ­l and loaded in another well for the identification of the proteins migration. GEL ELECTROPHORESIS : Cover the mini tank with lid properly by using colour code present on the banana plugs. Connect the gel electrophoresis tank to power supply by using 200volts of constant current for about 35-40 minutes until samples runs more than 3/4th of the gel. Stain and de-stain gels: After reaching the sample nearly bottom of the gel turn of the power supply to the gel electrophoresis tank and disconnect the electric leads. Discard the running buffer to avoid splitting and carefully remove the gel sandwich, gently separate the gel from plate by using sharp wedge, separated gel is placed in coomassie blue stain solution of 20-30 ml for 30 minutes on shaker for constant shaking. After the 30 minutes discard the stain solution and wash the gel with distilled water for 4- 5 times for constant time intervals and incubate at room temperature for overnight by placing on shaker. Finally rinse the detained gel with distilled water till the protein bands can clearly visible. Taken the pictures by using camera. RESULTS: By observing the obtained result after running the histone samples H4,H3.3,H2B,H2A in 1D SDS-PAGE . The separation of samples has been seen on the gel by using a dye coomassie blue stain solution. While observing the samples are run on gel according to their molecular mass and get separated from each other. The sample histone protein H4 shows little bit difference in observation which is present near to bottom of the gel ,that shows it run little bit faster than other histone samples due to its smaller size. While observing the other histone samples like H3.3,H2B,H2A they has no lot of difference in separation to differentiate from each other. DISCUSSION: The present experiment explains the isolation of human recombinant protein H4, H3.3, H2B and H2A by using the 1D SDS-PAGE. By observing the obtained result that found the histone protein H4 migrated little bit faster than the other samples .where as other histone samples H3.3, H2B, and H2A are observed ,there is no lot of difference in the migration to differentiate from each other. According to Kornberg, R.D when they performed the experiment on histone protein of human recombinant, found the histone protein H4 migrate faster than other protein and appears to be at 11 kDl . Where H3.3 appears near 15kDl,H2B appears near 14 kDl ,H2A appears near 12 kDl with these result we expect to be the same result but according to the above result that H3.3 ,H2B,H2A does not show much separation in migration of protein sample. So for getting such result may have many reasons that might have not loaded the samples with equal volume or properly loaded in the wells or power supply to the electrophor esis tank is not adjust properly or one sample over float into other wells while loading. The separation of the histone proteins that observed by different authors are histone proteins which undergoes non-acetylase that migrates faster than the protein that undergoes monoacetylation and acetylated derivatives. In this sequence the histone proteins are clearly separated in the core histone protein by using 1D SDS-PAGE. The retarded mobility are shown when the histone protein is highly acetylated compared with non acetylated parent compared. The variants are observed in histone protein due to differing of amino acids in the sequence. Histone proteins undergoes different biological conditions and form to be post synthetically modified like ADP-ribosylated, phosphorylated and acetylated .by using SDS-PAGE the purity of isolated proteins are identified. In the present experiment if the mixture of four histone proteins would need to be separated by the same technique. I would preferably take the three consequent results of same and expected as follows molecular size of H4 has l ess kDl than H2A, H2B and H3.3 in kDl. Few journals and reviews found to be support my hypothesis like Kornberg, R.D(1977) and Herbert and Linder(1992).

Effect Of Postimpressionists On The Next Generation Essay -- essays re

Postimpressionism Postimpressionism was a movement in late-19th-century French painting that emphasized the artist's personal response to a subject. Postimpressionism takes its name from an art movement that immediately preceded it: Impressionism. But whereas impressionist painters concentrated on the depiction of a subject's immediate appearance, postimpressionists focused on emotional or spiritual meanings that the subject might convey. Although impressionist artists interpreted what they saw, their approach nevertheless remained rooted in observation of the natural world. Postimpressionists conveyed their personal responses to the world around them through the use of strong, unnatural colors and exaggeration or slight distortion of forms. Postimpressionism can be said to have begun in 1886, the year that French painter Georges Seurat exhibited Sunday Afternoon on the Island of La Grande Jatte (1884-1886), and to have ended in 1906, the year French painter Paul CÃ ©zanne died. British art critic Roger Fry, however, coined the term postimpressionism, in 1910 when he organized an exhibition of French paintings at the Grafton Galleries in London. Fry is said to have been dissuaded from using the word expressionist to describe the work of CÃ ©zanne, Seurat, Paul Gauguin, Henri Matisse, Vincent van Gogh, and others, and to have finally declared: "Oh, let's just call them post-impressionists; at any rate, they came after the impressionists." The term was firmly established when Fry held a second show of postimpressionist art at the Grafton Galleries in 1912. The Postimpressionists The painters most closely associated with postimpressionism all took part in Fry's first exhibition: CÃ ©zanne, Seurat, Gauguin, Matisse, and van Gogh. Although their styles differed greatly from one another, these artists shared an ability to communicate concepts, emotions, or personal sensation through their art. Unlike other postimpressionists, Paul CÃ ©zanne did not create symbolic equivalents between elements of his paintings and particular emotions or concepts. Instead, CÃ ©zanne, who began his career as an impressionist, felt that he could communicate the intensity of his personal sensation through his painted observations of nature. He repeatedly turned to traditional artistic subjects, such as landscapes, still lifes, and nude bathers. However, his r... ...m, used more decorative shapes, stencilling, collage, and brighter colors. It was then that artists such as Picasso and Braque started to use pieces of cut-up newspaper in their paintings. An early 20th-century school of painting and sculpture in which the subject matter is portrayed by geometric forms without realistic detail, stressing abstract form at the expense of other pictorial elements largely by use of intersecting often transparent cubes and cones. CÃ ©zanne influenced cubism, the highly influential visual arts style of the 20th century that was created principally by Picasso and Braque in Paris between 1907 and 1914. The Cubist style emphasized the flat, two-dimensional surface of the picture plane, rejecting the traditional techniques of perspective, foreshortening, modeling, and chiaroscuro and refuting time-honoured theories of art as the imitation of nature. Cubist painters were not bound to copying form, texture, colour, and space; instead, they presented a new reality in paintings that depicted radically fragmented objects, whose several sides were seen simultaneously. this has been collected from various resources on the net ... ibiblio.org, among others

Home Depot’s Organizational Culture Essay

In my opinion, Nardelli’s programs were failed in changing operation of Home Depot to a more effective one. There are two reasons that Nardelli was not more successful. First, most employees did not want to an outsider to â€Å"GE-ize their company and culture. † Employees in Home Depot did not trust their new manager, which led absent support when Nardelli carried out the new process. The second reason was also the more important reason. Nardelli used the concept learned from industrial businesses to conduct the customer businesses. Since Nardelli wanted to conduct Home Depot in his own approach, he failed to recognize the importance of front-line staff, which leaded his fail in conducting the retailing business. The thing he should have done was improve the circulation of information between the different stores or within different level. Only in this way, he could solve the problem that Home Depot faced at that time—too large to be profitable. The worst thing he should not have done was to get rid of many old employees and replaced the full timers with part-time staff. This decline in the quality of customer service and customer satisfaction strongly hurt the customer orientated business. 2. Nardelli provided a three-part strategy including improving Home Depot’s profit, and expanding the business and market. Crow focused on the other three priorities which would revive employees’ sense of ownership, foster their product knowledge, and boost their morale. The greatest difference was that Nardelli’s initiatives programs were concentrated on increasing profit and expansion, while Tim Crow’s programs were concentrated on increasing employees. Nardelli’s action decreased employees’ satisfaction and loyalty, which ruined Home Depot’s strong customer service culture. Tim Crow’s programs increased employees’ loyalty and revived Home Depot’s organizational culture. 3. Strong culture reflects the values of the organization’s founders. Home Depot’s founders: Bernard Marcus and Arthur Black, as entrepreneurs, emphasized on commitment to customers, colleagues, and company. These conducted the original culture of Home Depot. Sometimes, the organizational culture could cause conflict when top management changed. When Nardelli toke over Home Depot, clash of two distinct cultural approaches occurred. Organizational culture that is consisted of shared beliefs, values, and assumptions existed in the organization would lead the behavior of employees and the direction of organization. Home Depot’s original culture let employees to establish strong relationship with customers, but after Nardelli’s conduction, this relationship as well as employees’ and loyalty were harmed. Since the culture would affect employees’ performance and organization’s achievement, it should be built to fit the characteristics of the business and altered with great care. Coordination, conflict resolution, and financial success were the assets of maintaining culture. However Nardelli failed to achieve these goals. Tim Crow, on the other hand, focused on restoring the initial culture by implementing more employee award programs that would positively impact employee loyalty and performance.

Society and Rules

Expository piece Carla Franz While the rules of society are meant to protect us, they exclude some individuals. ‘ As humans grow up into parts of society, they are connected by the rules which guide them in their thoughts and actions. However, as time progresses and societies change, many individuals are excluded due to new rules that surpass them. Stand by me demonstrates the only do laws change over time, but the societies that dictate them change as well.These rules are changed by the people who set examples to the est. of the world; a powerful example is the classic film ‘Stand by me' which demonstrates the will of the four protagonists who are excluded from their society. Gourde, Chris, Teddy and Fern are the main characters that have negative relationships and past with their family; this had a major impact on their segregation from their surrounding society.A key scene from the film is the conversation between Gourde and Chris; they state how they acknowledge the w eirdness in each other but also agreed that ‘everyone is weird'. This is one of the many prime examples how any individuals are excluded due to there weirdness and failure of following others in ‘society. Many individuals have been excluded from society over the years. Especially as young adolescents, we can become immersed into the rules of society.A well known quote is ‘all actions have benefits or consequences'. This saying is demonstrated in many of today's societies. Being individuals, if you do not fit in or follow societies rules you will be teased or looked at funny, people try to change themselves to fit in and live up to societies satisfaction. Author Glenda Millard explores this in A Small Free Kiss in the Dark. Skip one of the main protagonist's in the book who has Just progressed into his early adolescent years showing his innocent view of his world.Skip was an odd one at school, he wrote notes to his teacher making up excuses for not having friends; h e also invented other notes he would take to school to have permission to put his hands in the sky because that is how he finds about the light. One of the many excuses Skip has for not having friends is a simplistic note explaining that ‘he isn't good at math, the others might think he was dumb'. The note Skip wrote is telling us firstly, skip is scared he will be Judged by society because he is not good at math's and portrayed as ‘dumb', secondly he was often excluded at school because he didn't like the thought of having a friend.This is another example of how young individuals are excluded from society due to not being the smartest in his class or not wanting to associate with other kid's at school. Political exclusion is another major form of being excluded in society. Being denied the basic right to participate way in which society excluded the four protagonists, because of their past, showing the audience the brutal ways in which many individuals are excluded.A Sm all Free Kiss in the Dark also powerfully demonstrates how the four protagonists often grapple with their past and come together as excluded individuals of their own society. People come up with new rules to fit the changing society surrounding them, from the things people wear to the things people eat. As time has shown the changing says society operates in, it also bares the individuals that are excluded because of the rules that are ever changing.Not in who runs the country and how it is run has affected the human race since the creation of communities. North Korea, Russia and Syria are Just a few of the many countries in the world who have dictatorship rulers, this form of commands the rules in which their society runs by. Some of the rules set forth by the surrounding societies in Arabic countries have a substantial effect on the population, rules such as having a male accomplice when in public can make the male population feel excluded from there society.As previously mentione d the political exclusion affects the surrounding societies substantially, making some individuals feel excluded and left out. The rules of society are meant to protect and guide us in greater communities. Some of the rules exclude individuals, because of religion, fashion and in political ways. People try to change themselves to fit in society, because if you don't blend in with society you will be looked at differently and simply be excluded.